A TaqI RFLP at the gas-2 locus

TaqI RFLP at D21S137.

Chromosomal Localization: The 3 polymorphic bands were mapped to chromosome 22 using a somatic cell hybrid panel. The 2.1 kb constant band mapped to chromosome lq whereas all other constant bands mapped to chromosome 20. Whereas the active gene for hTOPl is located on chromosome 20ql 1.2—13.1, two pseudogenes are on chromosomes Iq23—24 and 22qll.2-13.1 (2). The sequences of hTOPl and the two ps...

A TaqI RFLP identified at the retinoblastoma locus on chromosome 13.

SOURCE/DESCRIPTION: Probe D95HS0.5 is a 0.6 kb fragment subcloned into Blue-scribe a pUCl9 derivative from a bacterio phage library isolated by a cDNA probe of the retinoblastoma gene 1. The fragment is released with the enzymes Hindlll and Sail. POLYMORPHISMS: TaqI identifies a 2 allele polymorphism with a band at 2.1 kb and 1.8 kb with a frequency of 0.97 and 0.03 respectively. There are no c...

Asp700 RFLP at the D8S45 locus.

Source/Description of Clone: Clone pALP13-8 is a single copy 750 bp EcoRV fragment, 0.9 kb 3' of exon 4, subcloned from a 6.6 kb BamHI fragment containing the complete human c-fos oncogene (FOS) (1). Polymorphisms: are detected with TaqI (A alleles) and BstNI (B alleles) (see Figure). A) TaqI: Presence of a 1.6 kb fragment or its allele, a 0.6 kb fragment. B) BstNI: Presence of an 0.85 kb fragm...

A TaqI RFLP for the human arylsulfatase A gene.

A BstE II RFLP at the DXS153 locus.

Source/Description: cpX12 is a 1.1 kb EcoRI single-copy genomic fragment cloned into pAT153 from a pool of 100 cosmids isolated from a human/hamster somatic cell hybrid containing only the human X chromosome (1). Polymorphism: cpX12 reveals a two-allele restriction fragment length polymorphism with bands at 0.7 kb and 1.9 kb (Al) or 2.6 kb (A2) after Southern blot analysis of human genomic DNA ...